Background to this app

This app displays data described in Lynch et al. The EMBO Journal, In Press (link below) - sorted bulk RNA-seq from hESC cardiac differentiations.

Cell model info

Samples were from HES3 hESCs on day 7.5 of mesendoderm differentiation as 3D embryoid bodies. Populations were sorted by FACS based on NKX2-5-GFP and SOX17-Tom reporter activity. Samples were process immediately for RNA-seq. 3 biological replicates were performed.

Plot info ('Data' tab)

The plot shows the normalized (DESeq) RNA expression level in each population. The boxplots follow standard Tukey representations.

Data download info

The 'download' tab includes option to download the data as a DESeqDataSet.

Raw FASTQ data files will soon be available via ArrayExpress E-MTAB-14276.

Reference

HAND1 level controls the specification of multipotent cardiac and extraembryonic progenitors from human pluripotent stem cells.

Lynch T, Phillips N, Douglas M, Dorgnach M, Lin H, Adamson AD, Darieva Z, Whittle J, Hanley NA, Bobola N, Birket MJ

The EMBO Journal, In Press

https://doi.org/10.1038/s44318-025-00409-0

Funders

Download options

Download RNA-seq dataset (DESeqDataSet)

Download1

Raw FASTQ data files will soon be available via ArrayExpress E-MTAB-14276:

https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-14276

Reference

The level of HAND1 controls the specification of multipotent cardiac and extraembryonic progenitors.

Lynch T, Phillips N, Douglas M, Dorgnach M, Lin H, Adamson AD, Darieva Z, Whittle J, Hanley NA, Bobola N, Birket MJ

bioRxiv 2024.08.15.607916

https://doi.org/10.1101/2024.08.15.607916